3. CRISPR

1. title page
2. tldr
3. part 1a
4. part 1b
5. Mechanism pt 1
6. Mechanism pt 2
7. Applications pt 1
8. Applications pt 2
9. Ethics

References*

  1. What is CRISPR-Cas9? (2016).
  2. What are genome editing and CRISPR-Cas9? MedlinePlus Genetics (2020).
  3. Ishino, Y., Krupovic, M., & Forterre, P. History of CRISPR-Cas from Encounter with a Mysterious Repeated Sequence to Genome Editing Technology. American Society for Microbiology (2018).
  4. CRISPR Timeline. Broad Institute (2018).
  5. Ledford, H., & Callaway, E. Pioneers of revolutionary CRISPR gene editing win chemistry Nobel. Nature (2020).
  6. Thurtle‐Schmidt, D. M. & Lo, T. W. Molecular biology at the cutting edge: A review on CRISPR/CAS9 gene editing for undergraduates. (2018).
  7. CRISPR Guide. addgene.
  8. Pak, E. CRISPR: A game-changing genetic engineering technique. Harvard University (2014).
  9. Hsu, P. D., Lander, E. S., & Zhang, F. Development and applications of CRISPR-Cas9 for genome engineering. Cell (2014).
  10. Voss, J. E. et al. Reprogramming the antigen specificity of B cells using genome-editing technologies. eLife (2019). 
  11. Yin, C. et al. In Vivo Excision of HIV-1 Provirus by saCas9 and Multiplex Single-Guide RNAs in Animal Models. Molecular Therapy (2017).
  12. Ledford, H. (2020, March 5). CRISPR treatment inserted directly into the body for the first time. Nature News (2020).  
  13. Gantz, V. M. et al. Highly efficient Cas9-mediated gene drive for population modification of the malaria vector mosquito Anopheles stephensi. PNAS (2015).
  14. Shwartz, M. CRISPR is a gene-editing tool that’s revolutionary, though not without risk. Stanford Medicine (2018).
  15. Shifrut, E. et al. Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function. Cell (2018).  
  16. Brokowski, C., & Adli, M. CRISPR Ethics: Moral Considerations for Applications of a Powerful Tool. JMB (2018).
  17. Human genome editing: science, ethics, and governance. The National Academies Press (2017).

*: We've formatted all references according to Nature format but left the links out. Instead, we've embedded them for your viewing pleasure!